C (3-6 months) Heterozygote, Hemizygote x Wild-type [C57BL/6JJcl] Heterozygote, Hemizygote x Wild-type [C57BL/6JJcl] 大阪大学微生物病研究所・竹田潤二、木下タロウ（1997）。R1 ES細胞を用いて作出。C57BL/6Jへ戻し交配された（N10以上）。 Cre/loxP system B6.129-Piga<tm1> B6.129-Piga<tm1> C（3〜6か月） Piga gene floxed mice. A loxP site was inserted between exons 5 and 6, and a loxP flanked Tk-neo cassette was inserted after the exon 5. <a href='https://brc.riken.jp/mus/pcr06211'>Genotyping protocol -PCR-</a> R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] Mouse Models for Human Disease true Taroh KINOSHITA Piga遺伝子のfloxedマウス。エクソン6がloxPで挟まれている (下流にloxPで挟まれたTk-neoカセットを持つ) 。完全に欠損したマウスの雄は胎生期9日目で致死。モザイクの雌は胎生14日頃で致死となり、頭部や顔面の形成異常がみられる。 Piga floxed, B6-Piga floxed Piga floxed, B6-Piga floxed Hematological Research Phage P1 loxP sites, herpes simplex virus thymidine kinase promoter (HSV tk promoter), E. coli neo, mouse Piga genomic DNA 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。<br>非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途MTAを締結すること。5年経過後も使用を希望するときは改めて寄託者から承諾を得るものとする。 The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Developed by Junji Takeda and Taroh Kinoshita, Research Institute for Microbial Diseases, Osaka University in 1997. R1 ES cells derived were used for the generation of floxed mice. The mutant mice were backcrossed to C57BL/6J over 10 times. 木下　タロウ RBRC06211